PrimerLab

real-time

A live co-pilot for PCR primer design. SantaLucia (1998) nearest-neighbor thermodynamics, secondary-structure scanning, and instant binding maps — every keystroke.

Target DNA Template0 bp

Forward primer0 nt

Reverse primer0 nt

Forward Tm

—

0 nt

Reverse Tm

—

0 nt

ΔTm

—

Awaiting primers

Amplicon

—

Forward not found in template

GC — Forward

—

Optimal 40–60%

GC — Reverse

—

Optimal 40–60%

Homopolymers — F

—

No runs >4

Homopolymers — R

—

No runs >4

Secondary structures

Hairpin

Clear · run 0

Self-dimer

Clear · run 0

Heterodimer

Clear · run 0

Binding map

Paste a target DNA template above to see the binding map.